sci论文

Antiproliferative effect of octreotide on gastric cancer cells

mediated by inhibition of Akt/PKB and telomerase

Abstract

AIM: To investigate the antiproliferative effect of octreotide,

a long-acting analogue of somatostatin, on gastric cancer

cell line SGC7901 and its possible molecular mechanisms.

METHODS: Gastric cancer cell line SGC7901 employed in

the study was treated with 0.008, 0.04, 0.2, 1, 5 and 25

mg·ml-1 of octreotide respectively for 24 h to evaluate the

antiproliferative effect of somatostatin analog on the tumor

cells by MTT assay method. To elucidate the underlying

mechanism, the cells were exposed to 1 mg·ml-1 of octreotide

for 0, 12, 24 and 48 h, when their Akt/PKB and telomerase

activities were respectively determined using PCR-ELSIA and

nonradioactive protein kinase assay protocols. The same

experimental procedures were also performed in the control

cells that were treated with corresponding vehicles instead

of somatostatin analog.

RESULTS: After exposed to octreotide for 24 h at the

concentrations of more than 1 mg·ml-1, SGC7901 cells

exhibited a dose-dependent inhibition of growth with the

inhibiting rate to be as high as 34.66 % when 25 mg·ml-1 of

octreotide was applied. The Akt/PKB and telomerase activity

of SGC7901 cells was significantly inhibited when the cells

were exposed to 1 mg·ml-1 of octreotide for 12, 24 and 48 h

compared with that of their control counterparts (P<0.01),< p="">

both of which exhibited in a time-dependent manner.

CONCLUSION: The antiproliferative effect of octreotide on

SGC7901 cells might be mediated by the inhibition of Akt/

PKB and telomerase.